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부품번호 SC-31155 기능
기능 EGFR (N-20)
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SC-31155 데이터시트, 핀배열, 회로
SANTA CRUZ BIOTECHNOLOGY, INC.
www.DataSheet4U.com
EGFR (N-20): sc-31155
The Power to Question
BACKGROUND
The EGF receptor family comprises several related receptor tyrosine kinases
that are frequently overexpressed in a variety of carcinomas. Members of
this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3)
and ErbB-4 (HER4), which form either homodimers or heterodimers upon lig-
and binding. Exons in the EGFR gene product are frequently either deleted or
duplicated to produce deletion mutants (DM) or tandem duplication mutants
(TDM), respectively, which are detected at various molecular weights. EGFR
binds several ligands, including epidermal growth factor (EGF), transforming
growth factor α (TGFα), Amphiregulin and heparin binding-EGF (HB-EGF).
Ligand binding promotes the internalization of EGFR via Clathrin-coated pits
and its subsequent degradation in response to its intrinsic tyrosine kinase.
EGFR is involved in organ morphogenesis and maintenance and repair of tis-
sues, but upregulation of EGFR is associated with tumor progression. The
oncogenic effects of EGFR include initiation of DNA synthesis, enhanced cell
growth, invasion and metastasis. Abrogation of EGFR results in cell cycle
arrest, apoptosis or dedifferentiation of cancer cells, suggesting that EGFR
may be an effective therapeutic target.
REFERENCES
1. Downward, J., Parker, P. and Waterfield, M.D. 1984. Autophosphorylation
sites on the epidermal growth factor receptor. Nature 311: 483-485.
2. Gullick, W.J., Downward, J. and Waterfield, M.D. 1985. Antibodies to the
autophosphorylation sites of the epidermal growth factor receptor protein-
tyrosine kinase as probes of structure and function. EMBO. J. 4: 2869-2877.
3. Gullick, W.J., Marsden, J.J., Whittle, N., Ward, B., Bobrow, L. and
Waterfield, M.D. 1986. Expression of epidermal growth factor receptors on
human cervical, ovarian, and vulval carcinomas. Cancer Res. 46: 285-292.
4. Berger, M.S., Gullick, W.J., Greenfield, C., Evans, S., Addis, B.J. and
Waterfield, M.D. 1987. Epidermal growth factor receptors in lung tumours.
J. Pathol. 152: 297-307.
CHROMOSOMAL LOCATION
Genetic locus: EGFR (human) mapping to 7p12; Egfr (mouse) mapping to
11 A2.
SOURCE
EGFR (N-20) is an affinity purified goat polyclonal antibody raised against a
peptide mapping within an N-terminal extracellular domain of EGFR of
human origin.
PRODUCT
Each vial contains 200 µg IgG in 1.0 ml of PBS with > 0.1% sodium azide
and 0.2% gelatin.
Blocking peptide available for competition studies, sc-31155 P, (100 µg
peptide in 0.5 ml PBS containing > 0.1% sodium azide and 0.2% BSA).
RESEARCH USE
For research use only, not for use in diagnostic procedures.
APPLICATIONS
EGFR (N-20) is recommended for detection of EGF receptor of mouse, rat and
human origin by Western Blotting (starting dilution 1:200, dilution range
1:100-1:1000), immunoprecipitation [1–2 µg per 100–500 µg of total protein
(1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution
range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution
range 1:30-1:3000).
Suitable for use as control antibody for EGFR siRNA (h): sc-29301 and EGFR
siRNA (m): sc-29302.
Molecular Weight of EGFR: 170 kDa.
Positive Controls: human breast carcinoma tissue, SK-BR-3 cell lysate:
sc-2218 or A-431 whole cell lysate: sc-2201.
RECOMMENDED SECONDARY REAGENTS
To ensure optimal results, the following support (secondary) reagents are
recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020
(dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey
anti-goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™
Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:
sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopre-
cipitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml).
3) Immunofluorescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution
range: 1:100-1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range:
1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941.
DATA
AB
211 K -
EGFR
118 K -
100 K -
EGFR (N-20): sc-31155. Western blot analysis of EGFR
expression in EGF-induced A-431 (A) and C3H/10T1/2
(B) whole cell lysates.
SELECT PRODUCT CITATIONS
1. Lai, M.D., et al. 2009. The effects of DNA formulation and administration
route on cancer therapeutic efficacy with xenogenic EGFR DNA vaccine in
a lung cancer animal model. Genet. Vaccines Ther. 7: 2.
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
PROTOCOLS
See our web site at www.scbt.com or our catalog for detailed protocols and
support products.
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com





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SC-31155

EGFR (N-20)

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